Diagnostic molecular techniques
₹ 10,000

Started on November 4, 2019 3 days

Recent advance in molecular diagnostic tools such as real-time PCR, next-generation sequencing has made disease detection rapid, affordable, accurate and portable. The course is aimed at training laboratory personnel who are interested in garner first-hand experience in molecular diagnostics aiming to improvise the existing molecular facility in their institution or even start a molecular diagnostic facility themselves. This course will cover basic concepts and techniques, standardization of tests and quality control in molecular diagnostics. The participants will have ample opportunity for hands-on training on several molecular assays in the diagnosis of various emerging infections.

    • Nucleic acid extraction from different clinical specimens
    • PCR, electrophoresis
    • cDNA synthesis, Real-time PCR, uniplex & multiplex PCR
    • Quantitative PCR
    • DNA sequencing
    • Application NGS in disease detection
    • POC molecular assay

This course is divided into three modules

  • Module 1 will present theoretical aspects of nucleic acid extraction from different clinical specimens, polymerase chain reaction including qualitative and quantitative polymerase chain reaction, real-time polymerase chain reaction (uniplex and multiplex).
  • Module 2 provides the practical experience to the concepts learnt in module 1.
  • Module 3 will focus on sequencing of nucleic acid, theory and applications of next-generation sequencing and point of care molecular assays.

At the end of the course, the partakers will be familiar with advanced molecular techniques and their application in modern day diagnostics.

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